robosep cell separating machine Search Results


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Robosep Automated Cell Separator, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Negative Selection Robosep Kit Easysep Human B Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Robosep Cd4 T Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Robosep Hla Easysep Wb Human T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc anti-cd138 magnetic-activated cell separation microbeads
Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Anti Cd138 Magnetic Activated Cell Separation Microbeads, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Robosep Automated Cell Separator, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Veritas Corporation magnetic cell separator robosep
Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Magnetic Cell Separator Robosep, supplied by Veritas Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc magnetic cell separator
Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Magnetic Cell Separator, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc robosep instrument
Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Robosep Instrument, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc robosep cell
Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Robosep Cell, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.

Journal: Diabetes

Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes

doi: 10.2337/db14-0929

Figure Lengend Snippet: Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.

Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

Techniques: Expressing, Generated, Fluorescence

Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.

Journal: Diabetes

Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes

doi: 10.2337/db14-0929

Figure Lengend Snippet: Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.

Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

Techniques: Expressing, Generated, Fluorescence